Osteoarthritis (OA) ke e 'ngoe ea mafu a nako e telele a sa foleng a sa foleng a amang batho ba tsofetseng ho feta lilemo tse 65.
1]. Ka kakaretso, bakuli ba OA ba fumanoa ba e-na le lefufuru le senyehileng, synovium e ruruhileng, le li-chondrocyte tse senyehileng, tse bakang bohloko le khatello ea 'mele [
2]. Bohloko ba ramatiki bo bakoa haholo ke ho senyeha ha lefufuru manonyeletsong ka lebaka la ho ruruha, 'me ha lefufuru le senyehile haholo masapo a ka thulana a baka bohloko bo ke keng ba mamelleha le mathata a 'mele [
3]. Ho kenya letsoho ha baemeli ba ho ruruha ba nang le matšoao a kang bohloko, ho ruruha le ho satalla ha lenonyeletso ho ngotsoe hantle. Ho bakuli ba OA, li-cytokine tse ruruhileng, tse bakang khoholeho ea lefufuru le lesapo la subchondral li fumanoa ka har'a mokelikeli oa synovial [
4]. Litletlebo tse peli tse kholo tseo bakuli ba OA hangata ba nang le tsona ke bohloko le ho ruruha ha synovial. Ka hona, sepheo sa mantlha sa liphekolo tsa hona joale tsa OA ke ho theola bohloko le ho ruruha. [
5]. Leha litlhare tse teng tsa OA, ho kenyeletsoa le lithethefatsi tse seng tsa steroidal le steroidal, li ipakile li sebetsa ho kokobetsa bohloko le ho ruruha, ts'ebeliso ea nako e telele ea lithethefatsi tsena e na le litlamorao tse mpe tsa bophelo bo botle joalo ka ho se sebetse hantle ha pelo, mala le mala.
6]. Kahoo, ho lokela ho etsoa moriana o sebetsang haholoanyane o nang le litla-morao tse fokolang bakeng sa ho phekola lefu la masapo a manonyeletso.
Lihlahisoa tsa tlhaho tsa bophelo bo botle li ntse li tsebahala haholo ka ho sireletseha le ho fumaneha habonolo [
7]. Meriana ea setso ea Korea e ipakile e sebetsa khahlano le mafu a 'maloa a ho ruruha, ho kenyelletsa ramatiki [
8]. Aucklandia lappa DC. e tsebahala ka melemo ea eona ea ho phekola, joalo ka ho matlafatsa phallo ea qi bakeng sa ho imolla bohloko le ho kokobetsa mala, 'me e sebelisitsoe ka tloaelo joalo ka setlhare sa tlhaho [
9]. Litlaleho tse fetileng li fana ka maikutlo a hore A. lappa e na le anti-inflammatory [
10,
11], analgesic [
12], anticancer [
13], le ho thibela malwetse [
14] liphello. Mesebetsi e sa tšoaneng ea likokoana-hloko ea A. lappa e bakoa ke metsoako ea eona e meholo e sebetsang: costunolide, dehydrocostus lactone, dihydrocostunolide, costuslactone, α-costol, saussurea lactone le costuslactone [
15]. Liphuputso tsa pejana li bolela hore costunolide e bontšitse thepa e khahlanong le ho ruruha ho lipopolysaccharide (LPS), e ileng ea susumetsa macrophages ka taolo ea NF-kB le tsela ea protheine ea mocheso [
16,
17]. Leha ho le joalo, ha ho boithuto bo entseng lipatlisiso ka mesebetsi e ka bang teng ea A. lappa bakeng sa kalafo ea OA. Patlisiso ea hajoale e batlisitse litlamorao tsa kalafo ea A. lappa khahlano le OA ka ho sebelisa (monosodium-iodoacetate) MIA le mefuta ea litoeba tse hlahisoang ke acetic acid.
Monosodium-iodoacetate (MIA) e sebelisoa ka mokhoa o tummeng ho hlahisa mekhoa e mengata ea bohloko le likarolo tsa pathophysiological tsa OA liphoofolong.
18,
19,
20]. Ha e kenngoa manonyeletsong a mangole, MIA e senya metabolism ea chondrocyte 'me e baka ho ruruha le matšoao a ho ruruha, a kang lefufuru le subchondral bone erosion, matšoao a mak'hadinale a OA [
18]. Karabelo ea writhing e bakoang ke asiti ea acetic e nkoa e le papiso ea bohloko ba liphoofolo moo bohloko ba ho ruruha bo ka lekanngoang ka bongata [
19]. The mouse macrophage cell line, RAW264.7, e atisa ho sebelisoa ho ithuta likarabo tsa cellular ho ruruha. Ha e sebelisoa le LPS, li-macrophages tsa RAW264 li kenya tšebetsong litsela tsa ho ruruha 'me li boloka li-intermediaries tse' maloa tsa ho ruruha, tse kang TNF-α, COX-2, IL-1β, iNOS, le IL-6 [
20]. Phuputso ena e hlahlobile liphello tse khahlanong le nociceptive le tse khahlanong le ho ruruha tsa A. lappa khahlanong le OA ka mohlala oa liphoofolo oa MIA, mohlala oa liphoofolo tse entsoeng ka acetic acid, le lisele tsa RAW264.7 tse entsoeng ka LPS.
2. Lisebelisoa le Mekhoa
2.1. Semela sa Semela
Motso o omisitsoeng oa A. lappa DC. e sebelisitsoeng tekong e ile ea rekoa ho Epulip Pharmaceutical Co., Ltd., (Seoul, Korea). E ile ea khetholloa ke Prof. Donghun Lee, Lefapha la Herbal pharmacology, Col. of Korean Medicine, Gachon University, 'me nomoro ea mohlala oa voucher e ile ea kenngoa e le 18060301.
2.2. HPLC Analysis of A. lappa Extract
A. lappa e ile ea ntšoa ho sebelisoa lisebelisoa tsa reflux (metsi a hloekisitsoeng, 3 h at 100 °C). Tharollo e nkiloeng e ne e tlhotliloeng le ho koaheloa ho sebelisoa mouoane o nang le khatello e tlase. A. lappa extract e fumane poelo ea 44.69% ka mor'a ho omisa ka tlas'a −80 °C. Tlhahlobo ea Chromatographic ea A. lappa e entsoe ka HPLC e hokahaneng ho sebelisoa 1260 InfinityⅡ HPLC-system (Agilent, Pal Alto, CA, USA). Bakeng sa karohano ea chromatic, kholomo ea EclipseXDB C18 (4.6 × 250 mm, 5 µm, Agilent) e sebelisitsoe ho 35 °C. Kakaretso ea 100 mg ea mohlala e ile ea hlapolloa ka 10 ml ea 50% methanol le sonicated bakeng sa metsotso e 10. lisampole li ne li tlhotliloeng ka filtara ea syringe (Waters Corp., Milford, MA, USA) ea 0.45 μm. Sebopeho sa karolo ea selefouno e ne e le 0.1% phosphoric acid (A) le acetonitrile (B) 'me kholomo e ile ea hlakoloa ka tsela e latelang: 0-60 min, 0%; 60-65 min, 100%; 65-67 mets, 100%; 67-72 min, 0% solvent B ka tekanyo ea phallo ea 1.0 mL / min. Mokelikeli o ile oa bonoa ho 210 nm ho sebelisoa molumo oa ente oa 10 μL. Tlhahlobo e entsoe ka makhetlo a mararo.
2.3. Bolulo le Tsamaiso ea Liphoofolo
Litoeba tsa Male Sprague-Dawley (SD) tse nang le libeke tse 5 le litoeba tsa banna tsa ICR tse nang le libeke tse 6 li rekiloe ho Samtako Bio Korea (Gyeonggi-do, Korea). Liphoofolo li ne li bolokiloe ka kamoreng li sebelisa mocheso o sa khaotseng (22 ± 2 ° C) le mongobo (55 ± 10%) le potoloho e khanyang / e lefifi ea 12/12 h. Liphoofolo li ne li tloaelane le boemo ka nako e fetang beke pele teko e qala. Liphoofolo li ne li e-na le phepelo ea li-ad libitum ea lijo le metsi. Melao ea morao-rao ea boitšoaro bakeng sa tlhokomelo ea liphoofolo le ho sebetsana le eona Univesithing ea Gachon (GIACUC-R2019003) e ne e lateloa ka thata mekhoeng eohle ea liteko tsa liphoofolo. Boithuto bo ne bo etselitsoe teko e foufalitsoeng ke mofuputsi le e tšoanang. Re ile ra latela mokhoa oa euthanasia ho latela litataiso tsa Komiti ea Boitšoaro ba Liphoofolo.
2.4. Ente ea MIA le Kalafo
Likhoto li ile tsa aroloa ka lihlopha tse 4, e leng sham, control, indomethacin, le A. lappa. Kaha li ne li e-na le anesthetized ka motsoako oa 2% oa isofluorane O2, likhoto li ne li kenngoa ka 50 μL ea MIA (40 mg / m; Sigma-Aldrich, St. Louis, MO, USA) intra-articularly ka har'a manonyeletso a mangole ho lebisa ho OA ea liteko. Liphekolo li ne li etsoa ka tlase: lihlopha tsa taolo le sham li ne li bolokoa feela ka lijo tsa motheo tsa AIN-93G. Feela, sehlopha sa indomethacin se ile sa fuoa indomethacin (3 mg/kg) e kenyelletsoeng lijong tsa AIN-93G le sehlopha sa A. lappa 300 mg/kg se abetsoe ho ja AIN-93G e tlatsitsoeng ka A. lappa (300 mg/kg). Liphekolo li ile tsa tsoela pele ka matsatsi a 24 ho tloha letsatsing la ho kenngoa ha OA ka tekanyo ea 15-17 g ka 190-210 g boima ba 'mele letsatsi le leng le le leng.
2.5. Boima ba Boima
Ka mor'a ho kenngoa ha OA, ho lekanya boima ba 'mele ba maoto a ka morao a likhoto ho ile ha etsoa ka incapacitance-MeterTester600 (IITC Life Science, Woodland Hills, CA, USA) joalokaha ho reriloe. Kabo ea boima maotong a ka morao e baloa: boima ba ho jara boima (%).
